肌苷促进脑损伤后神经功能恢复的实验研究

目的:中枢损伤是目前致残率最高的疾病之一,肌苷对于神经损伤后功能恢复的促进作用已经成为研究热点,本研究拟建立一侧前肢瘫痪的大鼠脑外伤模型,证实肌苷治疗促进中枢损伤后上肢功能恢复的有效性,同时初步探索其机制。方法:建立一侧运动皮层冲击损毁的大鼠模型,通过肢体不对称实验、抓取实验等行为学观察证实其患侧上肢功能受损,后在实验组进行肌苷药物14天,观察28天内上肢功能的恢复情况,与对照组作对比,证实其行为学上的有效性,同时对损伤侧大脑进行顺行BDA染色,探索其内在机制。结果:通过28天的观察发现经过肌苷治疗的的实验组大鼠肢体不对称实验、抓取实验等行为学评分明显好于隐性对照组,顺行BDA染色证实其有促进损伤周围健存皮层突触再生和代偿的作用。结论:肌苷可以促进中枢损伤后大鼠残存神经元得突触再生,使其大脑能在最大程度上代偿其丧失的功能,该药物可能会成为一种新的中枢损伤治疗的前体药物。

Inosine promotes recovery of neural function after traumatic brain injury

Objective: Central neurologic injury is one of the most common causes of limbs disturbance.Nowadays inosine treat-ment becomes a new focus.In this study we use a rat model of traumatic brain injury to certify the curative effect of inosine and investi-gate the internal mechanism.Methods: We established a rat model of traumatic brain injury and check the functional use of forepaw by limb use dependent test and reaching test.Thus a 14d term of inosine treatment was applied to the experimental group.All the ra...