| 半导体激光辐照对拟微绿球藻的生物学效应 |
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| 引用本文： | 刘晓娟,姜宝楠,庄惠如,陈荣. 半导体激光辐照对拟微绿球藻的生物学效应[J]. 生物技术, 2011, 21(4). DOI: 10.3969/j.issn.1004-311X.2011.04.110 |
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| 作者姓名： | 刘晓娟 姜宝楠 庄惠如 陈荣 |
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| 作者单位： | 1. 工业微生物教育部工程研究中心、福建师范大学生命科学学院、福建省现代发酵技术工程研究中心,福建福州,350108
2. 福建师范大学物理与光电信息科技学院,福建福州,350007 |
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| 基金项目： | 福建省自然科学基金项目 |
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| 摘 要： | 目的:研究半导体激光对拟微绿球藻的生物学效应,进而利用激光诱变筛选高产优质藻株.方法:采用半导体激光辐照拟微绿球藻,辐照时间为l 0min、20min、30min.结果:诱变后藻的代谢物产量均有提高.结论:l0min、20min、30min辐照条件下,对拟微绿球藻细胞生长及代谢产物均有不同程度的促进作用,其中10min辐照组有利于拟微绿球藻的生长及色素的积累,分别比对照组提高了45.06％、86.59％、56.06％；但在20min剂量下更有利于胞外多糖、总多糖、蛋白质及油脂含量的积累,分别比对照组提高了67.7％、51.89％、19.16％、69.78％.
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| 关 键 词： | 半导体激光 拟微绿球藻 油脂 |
Semiconductor- laser on the Biological Effect of Nannochloropsis sp.YW0980 |
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| LIU Xiao-juan,JIANG Bao-nan,ZHUANG Hui-ru,CHEN Rong. Semiconductor- laser on the Biological Effect of Nannochloropsis sp.YW0980[J]. Biotechnology, 2011, 21(4). DOI: 10.3969/j.issn.1004-311X.2011.04.110 |
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| Authors: | LIU Xiao-juan JIANG Bao-nan ZHUANG Hui-ru CHEN Rong |
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| Affiliation: | LIU Xiao-juan1,JIANG Bao-nan2,ZHUANG Hui-ru1,CHEN Rong2(1.Engineering Research Center of Industrial Microbiology,Ministry of Education,Engineering Research Center of Fujian Modern Fermentation Technology,College of Life Sciences,Fujian Normal University,Fuzhou 350108,China,2.College of Physics and Optoelectronics Technology,Fuzhou 350007) |
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| Abstract: | Objective:The biological effect of Nannochloropsis sp.YW0980 was researched under semiconductor-laser-irradiation to screen high-yield and high-quality algae strains.Method:The algae was induced bysemiconductor-laser in the does of 10min,20min,30min.Result:The yield of metabolites was increased after mutagenesis.Conclusion:The growth and metabolites accumulation of the algae were promoted by all three dose of semiconductor-laser.The dose of 10min was favourable to the growth and pigment accumulation of the ... |
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| Keywords: | semiconductor-laser Nannochloropsis sp.YW0980 lipid |
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