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| 一种快速分离检测产脂肽类生物表面活性剂枯草芽孢杆菌的方法 | |
| 引用本文： | 王大威,张健,姜伟,张凤久.一种快速分离检测产脂肽类生物表面活性剂枯草芽孢杆菌的方法[J].生物技术通报,2011(9). |
| 作者姓名： | 王大威 张健 姜伟 张凤久 |
| 作者单位： | 1. 中海油研究总院,北京100027;海洋石油高效开发国家重点实验室,北京100027 2. 中国海洋石油总公司,北京,100027 3. 中国海洋石油有限公司,北京,100027 |
| 基金项目： | 国家级重大基础研究前期研究专项(2008ZX05024) |
| 摘 要： | 脂肽(Lipopeptide)是由枯草芽孢杆菌(Bacillus subtilis)等微生物产生的一类具有较强表面活性的生物表面活性剂.枯革杆菌磷酸泛酰巯基转移酶基因(afp)是枯草芽孢杆菌中参与脂肽代谢的功能性基因.采用sfp基因PCR对从环境中得到的一组产生表面活性剂的微生物进行筛选,结合Tricine-SDS-PAGE电泳对PCR结果呈阳性的菌蛛的代谢粗初提物进行检测,初步鉴定得到两株枯草芽孢杆菌.进一步利用16S rDNA序列的系统发育学分析确定这两种菌株为枯草芽孢杆菌,并利用TLC、HPLC鉴定其产物为脂肽类表面活性剂,从而建立了一套快速分离检测产生脂肽类生物表面活性剂的枯草芽孢杆菌方法. |
| 关 键 词： | 脂肽 枯草芽孢杆菌 sfp基因 Tricine-SDS-PAGE 电泳 |
Rapid Detection and Characterization of Surfactin-producing Bacillus subtilis |
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| Wang Dawei,Zhang Jian,Jiang Wei,Zhang Fengjiu.Rapid Detection and Characterization of Surfactin-producing Bacillus subtilis[J].Biotechnology Bulletin,2011(9). | |
| Authors: | Wang Dawei Zhang Jian Jiang Wei Zhang Fengjiu |
| Affiliation: | Wang Dawei1,2 Zhang Jian1,2 Jiang Wei3 Zhang Fengjiu4(1 CNOOC Research Institute,Beijing 100027,2 State Key Laboratory of Offshore Oil Exploitation,3China National Offshore Oil Corporation,4 China National Offshore Oil Corporation Ltd,Beijing 100027) |
| Abstract: | Surfactin is a kind of important biosurfactant that metabolizes by Bacillus subtilis,but now strain Bacillus subtilis reported was little for identify to Bacillus subtilis and surfactin is time-consuming and complex.We research the utility of using PCR of the sfp gene as a means of identifying Bacillus species that produce surfactin,and using Tricine-SDS-PAGE as a means of identifying surfactin,16S rDNA sequence analysis and HPLC in parallel to ensure that the PCR and Tricine-SDS-PAGE provided correct resul... |
| Keywords: | Surfactin Bacillus subtilis sfp gene Tricine-SDS-PAGE electrophoresis |
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