Mapping and analysis of phosphorylation sites: a quick guide for cell biologists |
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| Authors: | Noah Dephoure Kathleen L. Gould Steven P. Gygi Douglas R. Kellogg |
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| Affiliation: | University of California, Berkeley;aDepartment of Cell Biology, Harvard Medical School, Boston, MA 02115-5701;bDepartment of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232-2175;cDepartment of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064-1077 |
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| Abstract: | A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments. |
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