Three-dimensional co-culture microfluidic model and its application for research on cancer stem-like cells inducing migration of endothelial cells |
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| Authors: | " target="_blank">Yi Zhao Xiaoqing Yan Bo Li Ming Ke Sijia Chen Zhiling Xu Shaoxi Cai |
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| Institution: | 1.Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering,Chongqing University,Chongqing,China;2.College of Bioengineering,Chongqing University,Chongqing,China;3.Chinese-American Research Institute for Diabetic Complications,Wenzhou Medical University,Wenzhou,China;4.School of Education,Chongqing Normal University,Chongqing,China |
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| Abstract: | ObjectivesTo build a three-dimensional co-culture model in a microfluidic device for cancer research and evaluate its feasibility by investigating cancer stem-like cells (SCs) induced migration of human umbilical vein endothelial cells (ECs).ResultsThe microfluidic device provided two-dimensional and three-dimensional (2D/3D) culture and co-culture environments without affecting cell viability. The device also provided an effective concentration for the chemiotaxis of cells, and to support real-time monitoring of cell behavior. In this model, SCs significantly increased the migration area of ECs with a hepatocarcinoma cell line (MHCC97H; MCs). The presence of ECs also induced both MCs and SCs invasion into Matrigel. The migration area of MCs and SCs significantly increased when co-cultured with ECs.ConclusionsThis 3D co-culture microfluidic model is a suitable model in cancer research. Compared with MCs, SCs had greater potential in inducing EC migration and interacting with ECs. |
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