Enhancement of 5-aminolevulinic acid production by metabolic engineering of the glycine biosynthesis pathway in <Emphasis Type="Italic">Corynebacterium glutamicum</Emphasis> |
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| Authors: | Yalan Zou Tao Chen Lili Feng Shuanghong Zhang Dongxu Xing Zhiwen Wang |
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| Institution: | 1.Department of Biochemical Engineering, School of Chemical Engineering and Technology,Tianjin University,Tianjin,People’s Republic of China;2.Key Laboratory of Systems Bioengineering (Ministry of Education),Tianjin University,Tianjin,People’s Republic of China;3.SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology,Tianjin University,Tianjin,People’s Republic of China;4.Sericulture and Agri-Food Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,People’s Republic of China |
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| Abstract: | ObjectiveTo construct a strain of Corynebacterium glutamicum capable of efficiently producing 5-aminolevulinic acid (5-ALA) via the C4 pathway by modification of serine and glycine pathway using glucose as sole carbon source.ResultsThe recombinant C. glutamicum strain AP2 harboring a codon-optimized hemA gene from Rhodobacter sphaeroides was used as host strain for 5-ALA production. A plasmid harboring the serine operon, which contained serB, serC and the site-specific mutant serA Δ197 , was constructed and introduced into C. glutamicumAP2, leading to an increase of 70% in 5-ALA production. Further overexpression of the glyA gene increased production of 5-ALA by 150% over the control. 5-ALA production was thus significantly enhanced by engineering the glycine biosynthetic pathway. C.glutamicum AG3 produced 3.4 ± 0.2 g 5-ALA/l in shake-flask cultures in CGIIIM medium with the addition of 7.5 g glycine/l.ConclusionThis is the first report of remodeling the serine and glycine biosynthetic pathway to improve the production of 5-ALA in C. glutamicum. |
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