A thermostable pyrimidine nucleoside phosphorylase from <Emphasis Type="Italic">Brevibacillus borstelensis</Emphasis> LK01 for synthesizing halogenated nucleosides |
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| Authors: | Ke Liu Youzhi Zhou Jinsong Zhang Jianlin Chu Yewei Zhang Bingfang He |
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| Institution: | 1.College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing,China;2.School of Pharmaceutical Sciences,Nanjing Tech University,Nanjing,China;3.Department of Hepatobiliary and Pancreatic Surgery, Zhongda Hospital, Medical School,Southeast University,Nanjing,China |
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| Abstract: | ObjectiveTo isolate a thermostable pyrimidine nucleoside phosphorylase (PyNP) from mesophilic bacteria by gene mining.Results BbPyNP from Brevibacillus borstelensis LK01 was isolated by gene mining. BbPyNP had a highest 60% identity with that of reported PyNPs. BbPyNP could catalyze the phosphorolysis of thymidine, 2′-deoxyuridine, uridine and 5-methyuridine. BbPyNP had good thermostability and retained 73% of its original activity after 2 h incubation at 50 °C. BbPyNP had the highest activity at an optimum alkaline pH of 8.5. BbPyNP was stable from pH 7 to 9.8. Under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reached the yield of 61–84%.ConclusionAn efficient approach was estimated in isolating thermostable PyNP from mesophilic bacteria. |
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