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Genetic improvement of Magnetospirillum gryphiswaldense for enhanced biological removal of phosphate
Authors:Yanping Zhou  author-information"  >,Wiktor Lisowski,Yan Zhou,Ng Wun Jern,Kama Huang,Eileen Fong
Affiliation:1.College of Electronics and Information Engineering,Sichuan University,Chengdu,China;2.Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute,Nanyang Technological University,Singapore,Singapore;3.School of Materials Science and Engineering,Nanyang Technological University,Singapore,Singapore
Abstract:

Objectives

To improve its phosphate accumulating abilities for phosphate recycling from wastewater, a magnetotactic bacterium, Magnetospirillum gryphiswaldense, was genetically modified to over-express polyphosphate kinase.

Results

Polyphosphate kinase was over-expressed in the bacterium. The recombinant strain accumulated ninefold more polyphosphate from synthetic wastewater compared to original wild type. The magnetic property of the recombinant M. gryphiswaldense strain was retained.

Conclusions

The recombinant M. gryphiswaldense can be used for phosphate removal and recovery in bioremediation.
Keywords:
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