Recovery of Bacillus thuringiensis from Marine Sediments of Japan |
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Authors: | Minoru Maeda Eiichi Mizuki Yukinobu Nakamura Toshitake Hatano Michio Ohba |
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Institution: | (1) Kyushu Medical Co., Ltd., 13-4 Ohte-machi, Kokurakita-ku, Kitakyushu, Fukuoka 803-0814, Japan , JP;(2) Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0816, Japan , JP;(3) Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan , JP |
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Abstract: | Marine sediments from a Japanese bay were examined for the occurrence of Bacillus thuringiensis. Of 1313 colonies belonging to the Bacillus cereus/B. thuringiensis group, 22 (1.7%) were allocated to B. thuringiensis. Marine isolates of B. thuringiensis consisted of heterogeneous multiple H serogroups; 10 isolates were assigned to the eight serovars (kurstaki, sumiyoshiensis, sotto, aizawai, darmstadiensis, thompsoni, neoleonensis, and higo); two motile isolates failed to react with the reference antisera; and the others were serologically untestable. Insecticidal
activities were associated with two kurstaki isolates (toxic to both Lepidoptera and Diptera) and a higo isolate (Diptera-specific). None of the parasporal inclusion proteins of the 22 isolates exhibited in vitro cytotoxic activity
against two vertebrate cells, sheep erythrocytes and HeLa cells. All B. thuringiensis isolates had no halophilism, although seawater-based medium supported their growth, sporulation, and formation of parasporal
inclusions.
Received: 29 November 1999 / Accepted: 10 January 2000 |
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