Bioreactor cultivation of Escherichia coli for production of recombinant penicillin G amidase from Alcaligenes faecalis |
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Authors: | Deak Peter M Lutz-Wahl Sabine Bothe Harald Fischer Lutz |
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Affiliation: | (1) Lehrstuhl für Biotechnologie, Institut für Lebensmitteltechnologie, Universität Hohenheim, Emil-Wolff-Straße 14, 70599 Stuttgart, Germany |
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Abstract: | The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter. Cells were grown in a synthetic minimal medium in a bioreactor (5 l working vol.), and production of PGA was induced by repeated addition of the inducer rhamnose, that served also as a carbon source. The fermentation yield was about 4500 units PGA activity per liter of culture medium. |
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Keywords: | Alcaligenes faecalis fermentation penicillin G amidase recombinant protein production rhamnose inducible promoter |
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