FACTORS INFLUENCING RESPIRATORY CONTROL IN BRAIN MITOCHONDRIA |
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| Authors: | E. S. Higgins |
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| Affiliation: | Department of Biochemistry, Medical College of Virginia, Richmond, Virginia and Bureau of Alcohol Studies and Rehabilitation, Department of Health, Commonwealth of Virginia |
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| Abstract: | A simple technique was described for isolation of the mitochondrial fraction from central nervous tissue of the rat. The fraction so prepared satisfied structural and functional criteria of intactness and relative homogeneity. Oxidation of various substrates and the influences of inhibitors and experimental conditions were examined. The refractoriness of brain mitochondria to thyroxine was confirmed. Oxidation of glutamate was characterized by a very high degree of respiratory control. There was a pronounced Mg requirement by mitochondria in the aerobic active state, but not in the controlled state. Reverse acceptor control was manifest during succinate oxidation by Mg deficient mitochondria. |
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