Characterization of ornamental Datura plants transformed by Agrobacterium rhizogenes |
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Authors: | Annalisa Giovannini Nicola Pecchioni Mario Rabaglio Andera Allavena |
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Institution: | (1) Istituto Sperimentale per la Floricoltura, C.so Degli Inglesi, 508, 18038 Sanremo (IM), Italy;(2) Istituto Sperimentale per la Cerealicoltura, Via S. Protaso, 302, 29017 Fiorenzuola d’Arda (PC), Italy |
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Abstract: | Summary
Datura arborea and D. sanguinea hairy roots were produced by cocultivation of leaf fragments with Agrobacterium rhizogenes strain NCPP 1855. Adventitious buds emerged spontaneously, without exogenous growth regulators, from seven hairy root clones
of D. arborea and from one hairy root clone of D. sanguinea. Regenerated plants were successfully acclimatized in the greenhouse. The integration of the bacterial TL-DNA into the genome
of the putative transformed plants was confirmed by Southern blot analysis. Transgenic plants displayed increased ability
to root in vivo. Morphological traits with relevant ornamental value like plant height, leaf number, size and shape, internode number, and
internode length were also affected. Transformation by wild-type Ri TL-DNA provided the chance to study plant growth and differentiation
and to select improved genotypes. |
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Keywords: | Brugmansia hairy roots ornamentals rol genes transgenic plants |
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