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Oxygen-derived radicals from Trypanosoma cruzi-stimulated human neutrophils |
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| Authors: | R Docampo A M Casellas E D Madeira R L Cardoni S N Moreno R P Mason |
| Institution: | 1. Centro de Investigaciones Bioenergéticas, Facultad de Medicina, Universidad de Buenos Aires, Argentina;2. Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina;3. Departamento de Inmunohematología, Academia Nacional de Medicina, Buenos Aires, Argentina;4. Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;5. National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA |
| Abstract: | This study provides biochemical and electron spin resonance spectroscopic evidence that contract of human polymorphonuclear leukocytes with antibody-coated Trypanosoma cruzi triggers the respiratory burst. Oxygen consumption, superoxide anion and hydrogen peroxide release were stimulated under conditions of polymorphonuclear leukocyte-mediated killing. This stimulation did not occur under non-killing conditions when antibody was omitted. A common mechanism of cytotoxicity of human polymorphonuclear leukocytes against different T. cruzi forms is suggested by the triggering of the respiratory burst by antibody-coated epimastigotes and trypomastigotes. |
| Keywords: | DMPO Neutrophil Hydrogen peroxide Free radical Superoxide Direct correspondence to R D and S N J M at present address: Laboratory of Environmental Biophysics National Institute of Environmental Health Sciences PO Box 12233 Research Triangle Park NC 27709 USA |
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