Isolation and monolayer culture of human fallopian tube epithelial cells |
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Authors: | Kazuhiro Takeuchi Ikuro Maruyama Sinichi Yamamoto Toshimichi Oki Yukihiro Nagata |
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Institution: | (1) Internal Medicine, Kagoshima University, Kagoshima, Japan;(2) Department of Obstetrics & Gynecology, Kagoshima University, Faculty of Medicine, 1208-1, Usuki-cho, 890 Kagoshima City, Japan |
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Abstract: | Summary In the present study we have established a pure monolayer culture system of human fallopian tube epithelial cells. The cells
were isolated using collagenase digestion, and were cultured in Medium 199 supplemented with 15% fetal bovine serum. The epithelial
cells derived from primary and secondary culture were characterized using immunocytochemical staining and electron microscopy.
The cells continued to grow for 2 to 3 wk once the monolayer culture of the cells was established. It is currently possible
to maintain the cultures until the third generation. Proliferation of these cells was enhanced by epidermal growth factor
but not by basic-fibroblast growth factor, insulin, transferrin, estradiol-17β, or progesterone. This culture system offers a good model for determining characteristics of the tubal epithelium and would
permit effective study of co-culture with embryos. |
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Keywords: | human tubal epithelium monolayer cell culture epidermal growth factor |
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