Identification of the rice D-genome chromosomes by genomic in situ hybridisation |
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| Authors: | K. Fukui R. Shishido T. Kinoshita |
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| Affiliation: | (1) Hokuriku National Agricultural Experiment Station, Joetsu 943-01, Japan Fax: +81-255-24-8578 Email: kfukui@inada.affrc.go.jp, JP;(2) Plant Breeding Institute, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan, JP;(3) Hokkaido University, Sapporo 060, Japan, JP |
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| Abstract: | The 24 rice D-genome chromosomes were identified among the 48 chromosomes of O. latifolia, which comprise the C- and D-genomes, using genomic in situ hybridisation (GISH). The B-genome chromosomes were also discriminated from the C-genome chromosomes in O. minuta (BBCC) by GISH. A comparison of the differences in the fluorescence intensity between the C and D genomes within O. latifolia (CCDD), and between the B and C genomes within O. minuta, indicated that the overall nucleotide-sequence homology between the B and C genomes is less than that between the C and D genomes. The origin of the D genome and the phylogenetic relationship of the D genome among the rice genomes are discussed, based on the results obtained. Received: 5 June 1997 / Accepted: 19 June 1997 |
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| Keywords: | Genomic in situ hybridisation (GISH) Genus Oryza O. latifolia (CCDD) O. minuta (BBCC) Rice D genome |
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