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A molecular genetic map of the long arm of chromosome 6R of rye incorporating the cereal cyst nematode resistance gene, CreR |
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| Authors: | C Taylor K W Shepherd P Langridge |
| Institution: | Department of Plant Biology, The Danish Institute of Agricultural Sciences, Research Center Flakkebjerg, Fors?gsvej 1, DK-4200 Slagelse, Denmark, DK Department of Plant Science, Waite Campus, University of Adelaide, South Australia 5064, Australia, AU ARC Special Research Centre for Basic and Applied Plant Molecular Biology, Department of Plant Science, Waite Campus, University of Adelaide, South Australia 5064, Australia, AU |
| Abstract: | A genetic map of the long arm of chromosome 6R of rye was constructed using eight homoeologous group-6 RFLP clones and five PCR markers derived from the rye-specific dispersed repetitive DNA family, R173. The map was developed using a novel test-cross F1 (TC-F1) population segregating for resistance to the cereal cyst nematode. Comparisons were made between the map generated with other rye and wheat group-6 chromosome maps by the inclusion of RFLP clones previously mapped in those species. Co-linearity was observed for common loci. This comparison confirmed a dramatic reduction in recombination for chromosome 6R in the TC-F1 population. The CreR locus was included in the linkage map via progeny testing of informative TC-F1 individuals. CreR mapped 3.7 cM distal from the RFLP locus, XksuF37. Comparative mapping should allow the identification of additional RFLP markers more closely linked to the CreR locus. Received: 14 April 1998 / Accepted: 29 April 1998 |
| Keywords: | CCN RFLP PCR Heterodera avenae Genetic mapping |
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