基于叶酸修饰的S-TiO2的PDT体外灭活HL60细胞实验研究

文章主要研究叶酸修饰硫掺杂二氧化钛(FA-S-TiO_2)纳米颗粒光催化灭活作用并初步探讨FA-S-TiO_2增强了TiO_2光催化灭活效率的作用机理。利用固相反应的方法制备了S-TiO_2,并利用表面修饰的方法制备了FA-S-TiO_2。通过紫外-可见光吸收光谱(UV-Vis),荧光光谱,傅里叶红外光谱(FTIR),透射电镜(TEM)等手段对样品进行了表征。利用Cell Counting Kit-8(CCK-8)法分别检测TiO_2、S-TiO_2、FA-S-TiO_2对HL60细胞的灭活效应,并采用活性氧检测等方法进行了分析研究。细胞实验结果表明,在暗室条件下,随着叶酸修饰S-TiO_2的比例增加,细胞的存活率随之减少;在光照条件下,细胞的存活率明显下降。其中FA-S-TiO_2样品1对HL60细胞的灭活效率最高,达到72%。其相应的活性氧含量也是最高。同时,通过荧光光谱推测FA-S-TiO_2提高HL60细胞对该纳米颗粒摄取效率,进而增强PDT灭活效果。

An Experimental Study on the Photocatalytic Inactivation of HL60 Cells in vitro Based on Sulfur-doped Titanium Dioxide Modified Folic Acid

The photocatalytic inactivation efficiency of HL60 cells in vitro based on sulfur-doped titanium dioxide modified folic acid (FA-S-TiO2) and its mechanism were researched in this paper.FA-S-TiO2 nanoparticles were synthesized by solid-phase reaction and surface modification method and were characterized by ultraviolet-visible absorption (UVVis),fluorescence spectra,fourier transformation infrared spectrum (FTIR),transmission electron microscope (TEM) and so on.The photocatalytic inactivation of these nanoparticles on HL60 cells was analyzed by CCK-8 assay and its mechanism was investigated using reactive oxygen species (ROS) detection.The results of cell experiment in the darkroom conditions showed that the viability of cells was reduced with the increment of FA.However,under the light exposure conditions,especially,FA-S-TiO2 sample 1 showed the highest cell inactivated efficiency of 72％,and the fluorescence intensity of the reactive oxygen species was the strongest.Meanwhile,the fluorescence spectra indicated that FAS-TiO2 may improve the photocatalytic inactivation by improving uptake efficiency of nanoparticles.