不同方法检测鸡蛋表面菌落总数的相关性研究

研究了ATP生物荧光检测法与国标法《GB4789.2-2010食品卫生微生物学检验菌落总数测定》检测鸡蛋壳表面细菌总数的相关性。采用ATP生物荧光检测法和国标法对40个混合样品表面细菌总数进行检测,以log CFU/个蛋壳为横坐标(x),以logRLU/个蛋壳为纵坐标(y),分别进行线性、对数、乘幂、指数拟合。结果表明,ATP荧光检测法与国标法检测结果 Pearson相关系数为0.912,线性模型y=0.7306x-1.0041(R2=0.8322)拟合度较高。该试验结果为ATP荧光检测法在鸡蛋壳表面细菌总数快速检测中应用的可行性提供了依据。

A Study on the Correlation of Different Methods for Detecting the Total Number of Bacteria on the Surface of Eggs

In this study,the bacterial count of the egg shell surface was compared by two methods: GB4789.2-2010 microbiological examination of food Hygiene: detection of aerobic bacterial count,ATP bioluminescence assay to study the correlation between ATP count and routine bacterial detection.The total bacteria count on the surface of 40 mixed samples was detected by ATP fluorescence detection,and the national standard method was used as the control.The Pearson correlation coefficient of ATP fluorescence test and the result of national standard method was 0.912.On the basis of these results,with log (CFU) as abscissa (x) and log (RLU) as ordinate (y),the linear fitting,logarithm fitting and exponent fitting were conducted.The fitting degree logarithmic model y=0.7306x-1.0041(R2=0.8322) was higher.ATP fluorescence detection method and the national standard method in the egg shell surface bacterial load detection results have a good correlation.ATP fluorescence detection can be replaced to some extent,routine testing of bacteria and applied to the actual production.