内皮抑素抗黑色素瘤血管新生时间窗及最佳剂量的研究

目的:获取不同黑色素瘤发展阶段的荷瘤鼠的最佳治疗时间以及最佳用药量。方法:将肿瘤大小不等的雄性黑色素瘤荷瘤鼠进行内皮抑素分组对比治疗,在用药3、5、7天后处死荷瘤鼠,剥离瘤体,称瘤重,分析生长趋势,做病理切片并进行HE染色和免疫组化。在与对照组进行对照后,根据肿瘤的大小、恶性化的程度以及CD31和VEGF的表达情况,找出内皮抑素的用药最有效的治疗时间窗。然后通过进一步实验,在已经检测出的不同肿瘤大小的荷瘤鼠的恩度作用时间窗内,给荷瘤鼠使用用不同浓度梯度的药物,在不同肿瘤发展大小之后剥离瘤体,做与上述类似的操作并分析,确定在治疗时间窗时的恩度使用的最佳剂量。结论:5天为内皮抑素抗黑色素瘤血管新生时间窗,两个实验组中,中等剂量即15 mg/kg和20 mg/kg为恩度作用最佳剂量。

Time Window and Optimum Quantity of Endostatin in Melanoma Angiogenesis Treatment

Objective:To get the exact appropriate time window and optimum medicine quantity in mice bearing melanoma treatment.Methods:Male mice bearing melanoma with different diameter were injected with endostatin for treatment,and were sacrificed separately 3,5,7 days after endostatin was applied.Then tumors were peeled,weighted,analyzed with growth trend and the pathological sections of the peeled tumors were analyzed by immunohistochemistry and HE staining.Compared with the control group,an appropriate time window for treatment would be determined with the size,grade of the tumors and expression of CD31 and VEGF considered.Next,under the condition of the known ideal window time,tumor-bearing mice were injected with endostatin at different concentrations.Similarly,an appropriate drug concentration would be found.Conclusion:The time 5 days after endostatin was applied was the exact appropriate time window in mice bearing melanoma treatment.And moderate doses,15mg/kg and 20mg/kg were the optimum endostatin quantities in this experiment.