Conjugal transfer of enterococcal transposons in Bacillus megaterium |
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| Authors: | M E Floccari B S Méndez |
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| Institution: | (1) Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina |
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| Abstract: | The conjugative enterococcal transposons Tn916 and Tn919 were introduced into Bacillus megaterium by a filtermating technique. The transfer frequencies obtained ranged from 1.3×10-6 to 6.6×10-7. The transposons integrated stably into the B. megaterium chromosome. Tn916 could generate auxotrophs and was transferred from B. megaterium Tn916 transconjugants to other species. |
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| Keywords: | Bacillus megaterium conjugative transposons Tn916 Tn919 |
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