| 虾夷扇贝遗传连锁图谱的初步构建 |
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| 引用本文： | 刘卫东,鲍相渤,宋文涛,周遵春,赫崇波,虞星炬. 虾夷扇贝遗传连锁图谱的初步构建[J]. 遗传, 2009, 31(6): 629-637. DOI: 10.3724/SP.J.1005.2009.00629 |
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| 作者姓名： | 刘卫东 鲍相渤 宋文涛 周遵春 赫崇波 虞星炬 |
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| 作者单位： | 1. 中国科学院大连化物所, 大连 116023;2. 辽宁省海洋水产科学研究院, 辽宁省海洋水产分子生物学重点实验室, 大连116023；;3. 辽宁师范大学, 生命科学学院, 大连 116023 |
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| 基金项目： | 国家海洋公益性行业科研专项基金，辽宁省海洋与渔业科研计划项目，辽宁省科学技术计划项目 |
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| 摘 要： | 用AFLP标记首次构建了虾夷扇贝遗传连锁图谱。用56对引物组合对父母本和52个F1代个体进行遗传连锁分析, 共得到1 855个标记, 其中多态位点为598(32.2%)个, 而354个符合孟德尔1: 1分离比。用这些标记和23个偏分离标记(0.01
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| 关 键 词： | AFLP标记 虾夷扇贝 遗传连锁图谱 |
| 收稿时间： | 2008-10-24 |
| 修稿时间： | 2008-12-24 |
The construction of a preliminary genetic linkage map in the Japanese scallop Mizuhopecten yessoensis |
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| LIU Wei-Dong,BAO Xiang-Bo,SONG Wen-Tao,ZHOU Zun-Chun,HE Chong-Bo,YU Xing-Ju. The construction of a preliminary genetic linkage map in the Japanese scallop Mizuhopecten yessoensis[J]. Hereditas, 2009, 31(6): 629-637. DOI: 10.3724/SP.J.1005.2009.00629 |
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| Authors: | LIU Wei-Dong BAO Xiang-Bo SONG Wen-Tao ZHOU Zun-Chun HE Chong-Bo YU Xing-Ju |
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| Affiliation: | 1. Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;2. Liaoning Ocean and Fisheries Science Research Institute, Liaoning Key Lab of Marine Fishery Molecular Biology, Dalian 116023, China;3. College of Life Science, Liaoning Normal University, Dalian 116023, China |
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| Abstract: | AFLP markers were used to construct the primary linkage map in a family of Mizuhopecten yessoensis. A total of 1 855 markers were generated in two parents and 52 progenies of the mapping family by using 56 AFLP primer combi-nations. Among the 1 855 markers, 598 were polymorphic and 354 were in agreement with the Mendelian segregating ratio of 1:1. Markers segregated according to Mendelian 1:1 ratio (P>0.05) and 23 distorted markers (0.01
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