Phospholipid and phospholipase changes by jasmonic acid during stolon to tuber transition of potato |
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Authors: | Ana Cenzano Renata Cantoro Graciela Racagni Cesar De Los Santos-Briones Teresa Hernández-Sotomayor Guillermina Abdala |
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Affiliation: | 1.Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales,Universidad Nacional de Río Cuarto,Rio Cuarto,Argentina;2.Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales,Universidad Nacional de Río Cuarto,Rio Cuarto,Argentina;3.Unidad de Bioquímica y Biología Molecular de Plantas,Centro de Investigación Científica de Yucatán, A. C. (CICY),Merida,Mexico |
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Abstract: | Potato tuber formation starts with the stolon swelling and is regulated by jasmonates. The cascade of events leading to tuber formation is not completely understood. The aim of this study was to evaluate phospholipid composition and phospholipase activities during four stages of stolon-to-tuber transition of Solanum tuberosum L., cv. Spunta, and involvement of phosphatidic acid (PA) in stolon cell expansion during early stages. Effects of jasmonic acid (JA) treatment on phospholipid content and activation of phospholipase D (PLD) (EC 3.1.4.4) and phosphatidylinositol-4,5-bisphosphate-specific phospholipase C (PIP2-PLC) (EC 3.1.4.3) were studied in the early stages (first stage, hooked apex stolon; second stage, initial swelling stolon) of tuberization. All the phospholipid species identified, phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), PA, and cardiolipin (CL), decreased as tuber formation progressed. PLD and PLC were activated in control tissues at an early stage. JA treatment caused a decrease of PC and PS in first stage stolons, accumulation of PA in second stage stolons, and modification of PLD and PLC activities. PA increased stolon cell area in the first and second stages. These findings indicate that phospholipid catabolism is activated from the early stages of tuber formation, and that JA treatment modifies the pattern of phospholipid (PC, PS, and PA) composition and phospholipase (PLD and PLC) activity. These phospholipids therefore may play a role in activation of an intracellular mechanism that switches the developmental fate of stolon meristem cells, causing differentiation into a tuber. |
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Keywords: | Jasmonic acid Phospholipids Phospholipases Solanum tuberosum Tuberization |
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