Asparagine-linked sugar chains of plasma membrane glycoproteins from healthy and common variable immunodeficiency B lymphoblastoid cell lines |
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Authors: | Naomi Kondo Fumiaki Motoyoshi Tadao Orii |
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Affiliation: | (1) Department of Pediatrics, Gifu University School of Medicine, Tsukasa-machi 40, 500 Gifu, Japan |
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Abstract: | Asparagine-linked sugar chains of plasma membrane glycoproteins, which are formed by glycosylation during B cell maturation, were examined with B lymphoblastoid cell lines (LCLs) transformed by Epstein-Barr virus derived from healthy controls and patients with common variable immunodeficiency (CVI). Both two patients with CVI showed hypogammaglobulinemia and impaired B cell functions. LCLs from healthy controls and the patients showed CD19+ and HLA/DR+ in the cell surface and secreted IgM. In both healthy controls and the patients, the main oligosaccharide in asparagine-linked sugar chains of the membrane glycoproteins of LCLs was biantennary sugar chain with bisected GlcNAc (Gal2-GlcNAc2-Man3-GlcNAc-GlcNAc-Fuc-GlcNAcOT). Biantennary sugar chain with an-fucosyl residue linked at the proximal GIcNAc was seen but biantennary sugar chain without an-fucosyl residue at the proximal GlcNAc was little detected in each LCL. There was no difference in quality and quantity of asparagine-linked sugar chains between healthy controls and the patients. These results suggest that glycosylation during B cell maturation may not be impaired in patients with CVI. |
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Keywords: | asparagine-linked sugar chain B lymphoblastoid cell lines common variable immunodeficiency glycosylation membrane glycoproteins |
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