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Modulation of immobilized enzyme activity by altering the hydrophobicity of nylon-grafted membranes: Part 1. Isothermal conditions |
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| Authors: | M M El-Masry A De Maio S Di Martino N Diano U Bencivenga S Rossi V Grano P Canciglia M Portaccio F S Gaeta D G Mita |
| Institution: | a International Institute of Genetics and Biophysics of CNR, via Guglielmo Marconi, 12, 80125 Naples, Italy b Department of Human Physiology and Integrated Biological Functions of the Second University of Naples, via S.M. di Costantinopoli, 16, 80138 Naples, Italy |
| Abstract: | The catalytic behaviour under isothermal conditions of two different membranes loaded with β-galactosidase was investigated. One membrane (M1) was constituted by a nylon sheet grafted with methylmethacrylate by means of chemical grafting. The other, (M2), was prepared by a double chemical grafting: the first one with styrene (Sty) and the second one with methylmethacrylate. Membrane activity was characterized as a function of temperature, pH and substrate concentration. The role of Sty in increasing membrane hydrophobicity has been discussed. Membrane M2 was found to be better suited for employment in non-isothermal bioreactors. |
| Keywords: | β-Galactosidase Grafted membranes Immobilized enzymes Lactose hydrolysis Bioreactors |
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