首页 | 本学科首页   官方微博 | 高级检索  
   检索      


The Effect of Modification of Nucleotide-37 on the Interaction of Aminoacyl-tRNA with the A Site of the 70S Ribosome
Authors:Soboleva  N G  Makhno  V I  Konevega  A L  Semenkov  Yu P  Katunin  V I
Institution:(1) Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad Region, 188300, Russia
Abstract:To estimate the effect of modified nucleotide 37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNAPhe +Y and Phe-tRNAPhe –Y) with the A site of complex 70S · poly(U) · deacylated tRNAPhe in the P site] was assayed at 0–20°C. As comparisons with native Phe-tRNAPhe +Y showed, removal of the Y base decreased the association constant of Phe-tRNAPhe –Y and the complex by an order of magnitude at every temperature tested, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNAPhe bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRNAPhe –Y but not for Phe-tRNAPhe +Y. Thus, the modified nucleotide 3" of the Phe-tRNAPhe anticodon stabilized the codon–anticodon interaction both in the A and P sites of the 70S ribosome.
Keywords:tRNAPhe  70S ribosome  A site  modified nucleotide  Y-base
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号