| 絮凝法预处理透明质酸发酵液的研究 |
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| 引用本文： | 吴华昌,马钦元,邓静,徐静,陈哲. 絮凝法预处理透明质酸发酵液的研究[J]. 生物技术, 2010, 20(1): 81-84 |
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| 作者姓名： | 吴华昌 马钦元 邓静 徐静 陈哲 |
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| 作者单位： | 四川理工学院生物工程系,四川,自贡,643000 |
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| 摘 要： | 目的:提高透明质酸的纯度。方法:研究了絮凝预处理发酵液对醇沉法提取透明质酸的影响,并通过正交试验对预处理条件进行了优化。结果:明矾可作为最佳絮凝剂,正交实验最佳絮凝条件为:絮凝剂添加量1 000mg/L、絮凝温度30℃、絮凝转速60r/min、絮凝时间20min。在该条件下处理的HA发酵液相对于未经过处理的对照组,菌体去除率可提高42.6%,蛋白去除率可提高5.9%。
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| 关 键 词： | 透明质酸 发酵液 分离提纯 絮凝 |
Study on the Pretreatment of Broth of Hyaluronic Acid |
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| WU Hua-chang,MA Qin-yuan,DENG Jing,XV Jing,CHEN Zhe. Study on the Pretreatment of Broth of Hyaluronic Acid[J]. Biotechnology, 2010, 20(1): 81-84 |
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| Authors: | WU Hua-chang MA Qin-yuan DENG Jing XV Jing CHEN Zhe |
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| Affiliation: | WU Hua-chang,MA Qin-yuan,DENG Jing,XV Jing,CHEN Zhe(Deptartment of Biotechnology Engineering,Sichuan University of Science & Engineering,Zigong 643000,China) |
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| Abstract: | Objective:Taking the improvement of the purity.Method:The effect of purification of Hyaluronic acid from broth by ethanol precipitation method was investigated by pretreating the broth using flocculants,and the factors were optimized by the orthogonal method.Result: Alum is the best flocculants.The best flocculating condition is got by orthogonal experiment.A conclusion could be got that the removal rate of cell could be improved by 42.6% and The removal rate of protein could be improved by 5.9% under the c... |
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| Keywords: | hyaluronic acid broth separation and purification flocculants |
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