Reversible sporicidal action of cuprous ions |
| |
Authors: | G C Rutherford R E Marquis |
| |
Affiliation: | (1) Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642-8672, USA, US |
| |
Abstract: | At 10 mM, Cu+ was highly protective against killing of spores of Bacillus megaterium ATCC 19213 by H2O2, while at higher concentrations, from 15–100 mM, killing was augmented. In contrast, Cu2+, Fe2+, Fe3+, Co2+ or Co3+ ions acted only protectively. Cu+ itself was sporicidal in the absence of H2O2 or ascorbate, and its sporicidal action did not depend on generation of highly reactive oxygen species. It appeared that killing involved either inhibition of germination or copper toxicity to germinated cells in that Cu+-inactivated spores did not germinate readily but chemical decoating of the cells prior to plating on a solid medium resulted in reversal of the sporicidal effect. Received 12 July 1996/ Accepted in revised form 03 November 1996 |
| |
Keywords: | : sporicides bacterial spores copper toxicity oxidative damage |
本文献已被 SpringerLink 等数据库收录! |
|