Bioconversion of acid-hydrolyzed poplar hemicellulose to acetic acid byClostridium thermoaceticum |
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Authors: | J. E. Brownell J. P. Nakas |
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Affiliation: | (1) Department of Environmental and Forest Biology, SUNY College of Environmental Science and Forestry, 13210 Syracuse, NY, USA |
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Abstract: | Summary Clostridium thermoaceticum was used to ferment carbohydrate released from pretreated oat splet xylan and hemicellulose isolated from hybrid poplar. Hydrolysis with dilute sulfuric acid (2.5% (v/v) for oat spelt xylan and 4.0% (v/v) for poplar hemicellulose) at 100°C for 60 min was found to release the highest concentration of fermentable substrate.C. thermoaceticum, when grown in non-pH controlled batch culture at 55°C under a headspace of 100% CO2, typically produced 14gl–1 acetic acid during a 48 h fermentation in medium containing 2% xylose. In fed-batch fermentations this organism was able to produce 42gl–1 acetic acid after 116h when the concentration of xylose was maintained at approximately 2% and the pH was controlled at 7.0. |
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Keywords: | Clostridium thermoaceticum Hemicellulose Thermophile Xylose Acetic acid |
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