The Lhcb protein and xanthophyll composition of the light harvesting antenna controls the DeltapH-dependency of non-photochemical quenching in Arabidopsis thaliana |
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| Authors: | Pérez-Bueno Maria L Johnson Matthew P Zia Ahmad Ruban Alexander V Horton Peter |
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| Affiliation: | Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom. |
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| Abstract: | Nonphotochemical quenching (NPQ) is the photoprotective dissipation of energy in photosynthetic membranes. The hypothesis that the DeltapH-dependent component of NPQ (qE) component of non-photochemical quenching is controlled allosterically by the xanthophyll cycle has been tested using Arabidopsis mutants with different xanthophyll content and composition of Lhcb proteins. The titration curves of qE against DeltapH were different in chloroplasts containing zeaxanthin or violaxanthin, proving their roles as allosteric activator and inhibitor, respectively. The curves differed in mutants deficient in lutein and specific Lhcb proteins. The results show that qE is determined by xanthophyll occupancy and the structural interactions within the antenna that govern allostericity. |
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| Keywords: | 9aa, 9-aminoacridine DES, de-epoxidation state of the xanthophyll cycle pool LHCII, light harvesting complexes of photosystem II Lhcb, the proteins of LHCII NPQ, non-photochemical quenching ΔpH, pH difference across the thylakoid membrane PSII, photosystem II q9aa, quenching of 9aa fluorescence qE, the ΔpH-dependent component of NPQ WT, wild-type zea, zeaxanthin |
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