Isolation and characterization of catabolite repression control mutants of Pseudomonas aeruginosa PAO. |
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| Authors: | J A Wolff C H MacGregor R C Eisenberg and P V Phibbs Jr |
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| Institution: | Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298. |
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| Abstract: | Independently controlled, inducible, catabolic genes in Pseudomonas aeruginosa are subject to strong catabolite repression control by intermediates of the tricarboxylic acid cycle. Mutants which exhibited a pleiotropic loss of catabolite repression control of multiple pathways were isolated. The mutations mapped in the 11-min region of the P. aeruginosa chromosome near argB and pyrE and were designated crc. Crc- mutants no longer showed repression of mannitol and glucose transport, glucose-6-phosphate dehydrogenase, glucokinase, Entner-Doudoroff dehydratase and aldolase, and amidase when grown in the presence of succinate plus an inducer. These activities were not expressed constitutively in Crc- mutants but exhibited wild-type inducible expression. |
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