Asymmetric somatic hybridization between tomato (Lycopersicon esculentum Mill) and gamma-irradiated potato (Solanum tuberosum L.): a quantitative analysis |
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| Authors: | H. C. H. Schoenmakers A. -M. A. Wolters A. de Haan A. K. Saiedi M. Koornneef |
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| Affiliation: | (1) Department of Genetics, Agricultural University Wageningen, Dreijenlaan 2, NL-6703 HA Wageningen, the Netherlands |
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| Abstract: | We analyzed 110 asymmetric fusion products, obtained by fusion of hygromycin-resistant tomato protoplasts and gamma-irradiated kanamycin-resistant potato protoplasts that expressed  -glucuronidase (GUS). The fusion products were selected for resistance to both antibiotics, and were subsequently analyzed for their shoot regeneration potential, GUS activity, expression of two potato isoenzymes, chloroplast type, total genomic DNA content, and relative genomic composition. No viable plants could be obtained and the calli were highly polypoid. All hybrids expressed GUS activity, whereas they displayed a large variation with respect to the other traits. |
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| Keywords: | Tomato Potato Asymmetric somatic hybridization DNA elimination Genome and plastome constitution |
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