Phosphate inhibits acetotrophic methanogenesis on rice roots |
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Authors: | Conrad R Klose M Claus P |
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Institution: | Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany. conrad@mailer.uni-marburg.de |
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Abstract: | The contribution of acetate- and H(2)/CO(2)-dependent methanogenesis to total CH(4) production was determined in excised washed rice roots by radiolabeling, methyl fluoride inhibition, and stable carbon isotope fractionation. Addition of > or = 20 mM phosphate inhibited methanogenesis, which then was exclusively from H(2)/CO(2). Otherwise, acetate contributed about 50 to 60% of the total methanogenesis, demonstrating that phosphate specifically inhibited acetotrophic methanogens on rice roots. |
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