Mild hydrolysis of nitriles byFusarium solani strain O1 |
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Authors: | V Vejvoda O Kaplan J Klozová J Masák A Čejková V Jirků R Stloukal L Martínková |
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Institution: | Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czechia. |
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Abstract: | High levels of an aromatic nitrilase (about 37 microkat/L culture) were induced in Fusarium solani O1 after transfer of the mycelium from a rich medium into a medium with 20 mmol/L picolinonitrile. The mycelium was entrapped in lense-shaped particles consisting of a polyvinyl alcohol/polyethylene glycol copolymer (LentiKats). The cell-free extract was immobilized by hydrophobic binding onto a Butyl Sepharose column. The enzyme was useful for the mild hydrolysis of nicotinonitrile, isonicotinonitrile and benzonitrile. |
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