我国首例肺微小根毛霉病及其致病菌的分离和培养

本文报告1例肺结核和糖尿病并发由微小根毛霉(Rhizomuror pusillus)所致的肺微小根毛霉病。首次在我国从病人的肺组织活检标本分离出该种真菌。此菌可在几种琼脂培养基20°—45℃条件下生长,最适温度为37℃。菌落在初期白色,然后变成褐色厚毡状。在光学显微镜下可看到孢囊梗假轴状分枝,初期无色,然后变为褐色。孢子囊直径50—90μm,呈灰色,后变为褐色。孢子囊成熟后囊壁消解。囊轴卵形或梨形,直径45—48μm。在有性期,接合孢子球形,直径43—63μm,初呈褐色,后变为黑色,表面粗糙或凹凸不平。在透射电子显微镜下,孢囊孢子呈不规则卵形,直径2—5μm;在扫描电子显微镜下,孢囊孢子形态与上述相同。本菌实验感染家兔、豚鼠和小白鼠显示毒力很强,动物于接种后3—10天内全部死亡。从感染动物的脏器分离出本菌。采用中、西医结合治疗,病人痊愈。

PULMONARY MUCORMYCOSIS CAUSED BY RHIZOMUCOR PUSILLUS,A NEW RECORD IN CHINA,AND ISOLATION AND CULTURE OF THE PATHOGEN

A patient suffering from the complex of pulmonary tubaculosis,diabetes, and pulmonary form of mueormycosis caused by Rhizomucor pusillus is reported.The pa- thogen was isolated from the lung tissues for the first time in China.It grows on agar media at 20℃—45℃.The optimal temperature is 37℃.The colonies were white in color at early stage then became brown.The pseudoxailly branched sporangiophores were observed under light microscope.At first,they were colorless then became brown.Sporangia are 50— 90μm in diameter,gray in color then turned brown.At maturity,the sporangial walls dis- appeared.The collumellae are ovoid or ellipsoid 45—48μm in diameter.Zygospores are glo- bular in shap,42—63μm in diameter.They were brown then became black with process on the surface.The sporangiospores are irregular ovoid in shap 2—5μm in diameter under transmission electron microscope (×10000).The same morphology of sporangiospore was ob- served under scanning electron microscope (×7700).This pathogen is very virulent to rabbit, guinea pig,and mice in experimental infection.Animals all died within 3—10 days after in- oculation.The same pathogen was isolated from the organs of infected animals.The patient was completely recovered after medicinal treatment.