Postsynthetic modification of oligonucleotides with imidazophenazine dye and its effect on duplex stability |
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Authors: | Dubey Larysa Ryazanova Olga Zozulya Victor Fedoryak Dmytro Dubey Igor |
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Institution: | Institute of Molecular Biology and Genetics, National Academy of Sciences, Kyiv, Ukraine. |
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Abstract: | Carboxyalkyl derivative of the intercalating agent imidazo4,5-b]phenazine was used for the postsynthetic oligonucleotide modification. Model pentadecathymidylate-imidazophenazine conjugate was prepared from 5'-aminoalkyl-modified (dT)(15) by using phosphonium coupling reagent BOP in the presence of 1-hydroxybenzotriazole. Spectral-fluorescent properties of the conjugate were studied. The attachment of the dye was found to increase the thermal stability of (dT)(15) duplex with poly(dA) by more than 4°C, probably by the intercalation mechanism. |
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