A high-throughput soft agar assay for identification of anticancer compound |
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| Authors: | Anderson Steven N Towne Danli L Burns David J Warrior Usha |
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| Affiliation: | Abbott Laboratories, Global Pharmaceutical Research and Development, Department of Biological Screening, Abbott Park, IL, USA. |
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| Abstract: | A 384-well soft agar assay was developed to identify potential novel anticancer compounds. Normally used to detect cell transformation, the assay is used here to quantitate cell proliferation in a 3-dimensional (3-D) anchorage-independent format. HCC827 cells, which are highly sensitive to epithelial growth factor receptor (EGFR) tyrosine kinase inhibitors, were used to develop the method and a set of 9600 compounds used to validate the assay. Results were compared to a monolayer assay using the same compound set. The assay provides a robust method to discover compounds that could be missed using traditional monolayer formats. |
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