Sensitive method for the quantitative determination of bromocriptine in human plasma by liquid chromatography-tandem mass spectrometry |
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Authors: | Salvador Arnaud Dubreuil Didier Denouel Jannick Millerioux L |
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Institution: | CEPHAC Europe, 90 Avenue des Hauts de la Chaume, BP 28, 86281 Saint-Benoit, France. arnaud.salvador@univ-lyon1.fr |
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Abstract: | A sensitive LC-MS-MS assay for the quantitative determination of bromocriptine has been developed and validated and is described in this work. The assay involved the extraction of the analyte from 1 ml of human plasma using a solid phase extraction on Oasis MCX cartridges. Chromatography was performed on a Symmetry C18 (2.1 mm x 100 mm, 3.5 microm) column using a mobile phase consisting of 25:75:01 acetonitrile-water-formic acid with a flow rate of 250 microl/min. The linearity was within the concentration range of 2-500 pg/ml. The lower limit of quantification was 2 pg/ml. This method has been demonstrated to be an improvement over existing methods due to its greater sensitivity and specificity. |
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