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Microsatellite markers isolated from barn swallows (Hirundo rustica)
Authors:OLGA V TSYUSKO  MAUREEN B PETERS  CRIS HAGEN  TRACEY D TUBERVILLE  TIMOTHY A MOUSSEAU  ANDERS P M LLER  TRAVIS C GLENN
Institution:OLGA V. TSYUSKO,MAUREEN B. PETERS,CRIS HAGEN,TRACEY D. TUBERVILLE,TIMOTHY A. MOUSSEAU,ANDERS P. MØLLER,TRAVIS C. GLENN
Abstract:Fifteen polymorphic microsatellite DNA loci were isolated from barn swallow (Hirundo rustica) and optimized for future studies of radiation‐induced mutations in populations from Ukraine. The loci were screened for variability among 25 individuals from two populations. The primers amplified loci with relatively high numbers of alleles ranging from five to 32 alleles per locus and polymorphic information content from 0.481 to 0.951. Observed heterozygosity varied from 0.458 to 0.960. None of the loci showed deviations from Hardy–Weinberg equilibrium in either population.
Keywords:Hirundo rustica  microsatellites  mutation  PCR  primer  radiation
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