细菌nox基因研究进展

细菌nox基因编码合成一种含核黄素的NADH氧化酶,NADH氧化酶可催化双原子氧还原为H2O2或H2O,同时将NADH氧化为NAD+。该反应发生在多种代谢途径中,从而对细菌的氧化应激、菌膜形成、毒力调控及代谢产物生成等生理生化过程产生一系列影响。目前对高等动植物体中的nox基因及其编码的NADH氧化酶已有较深入的研究,但近年来一些研究表明,细菌nox基因的功能及作用通路与动植物体存在较大差异,因此,有必要详细了解细菌中nox基因和NADH氧化酶的具体作用机制及其对细胞产生的影响。综合分析近年来细菌nox基因及NADH氧化酶的研究成果,结合我们的研究,对目前存在的问题和未来的发展进行综述。

Advances in Bacterial nox Gene

Encoded nox gene of bacteria synthesizes a riboflavin containing NADH oxidase, and NADH oxidase could catalyze the reduction of double-atom of oxygen into H₂O₂ or H₂O, simultaneously oxidize NADH into NAD⁺. This reaction occurs in various metabolic pathways, and accordingly has a series of effects on bacterial oxidative stress response, bacterial membrane formation, virulence regulation, metabolites production, as well as physiological and biochemical processes etc. At present there have been intensive studies on the nox gene and its encoded NADH oxidase in higher animals and plants, but recently some studies have demonstrated that the function and channel of action of nox gene in bacteria exists fairly difference from those in animals and plants. Therefore, it is necessary to understand about the specific mechanism of action of the NADH oxidase in bacteria and its effect on cells in detail. A comprehensive analysis on some outcomes of nox gene and NADH oxidase researches in bacteria achieved in recent years, combined with the studies in the universities, the existing problems, and its future development are reviewed and animadverted in this paper.