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Microsatellite markers isolated from saltgrass (Distichlis spicata)
Authors:OLGA V. TSYUSKO  MAUREEN B. PETERS  TRACEY D. TUBERVILLE  CRIS HAGEN  SARAH M. EPPLEY  TRAVIS C. GLENN
Abstract:Twelve polymorphic microsatellite DNA loci were isolated from saltgrass (Distichlis spicata) and optimized for future studies of its breeding system. The loci were screened for variability among 24 individuals from two populations. The primers amplified loci with numbers of alleles ranging from four to 14 per locus and polymorphic information content from 0.481 to 0.951. Observed heterozygosity varied from 0.227 to 0.958.
Keywords:breeding system  Distichlis spicata  microsatellites  mutation  PCR  primer
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