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Development of 14 multiplexed microsatellite loci for raccoons Procyon lotor
Authors:JENNIFER A. FIKE  ANDREA M. DRAUCH  JAMES C. BEASLEY  GUHA DHARMARAJAN  OLIN E. RHODES
Abstract:In order to complement ecological information with genetic data we isolated and characterized 14 polymorphic microsatellite markers from raccoons (Procyon lotor). Three multiplexed panels comprising the loci were developed and 29 individuals from a contiguous habitat patch in northern Indiana, USA were genotyped. The number of alleles per locus ranged from four to 18, and overall heterozygosities ranged from 0.31 to 1.00. One locus was identified as possibly being X‐linked, since males appeared to be hemizygous. Data generated using these markers will be used to further our understanding of small‐scale raccoon population dynamics in a highly fragmented landscape.
Keywords:microsatellite  multiplex PCR  Procyon lotor  raccoon
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