Involvement of alpha- and beta-PKC in the differentiation of 3T3-L1 cells |
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| Authors: | H Ueda Y Ishida H Taniguchi |
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| Institution: | Second Department of Internal Medicine, Kobe University School of Medicine, Japan. |
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| Abstract: | Present investigation revealed that 3T3-L1 cells contained two protein kinase C (PKC) subspecies, i.e. alpha- and beta-PKC. These cells treated by staurosporine, a specific inhibitor of PKC, differentiated to adipocytes more remarkably than those without its treatment. Their alpha- and beta-PKC activity decreased to 49% and 18% of the staurosporine-untreated cells respectively, and GPDH activity, a marker enzyme of adipocytes, increased to 143%. Thus, both alpha- and beta-PKC seemed to be associated with the adipose conversion in 3T3-L1 cells. |
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