Effects of COX-2 inhibitors on ROS produced by Chlamydia pneumoniae-primed human promonocytic cells (THP-1) |
| |
Authors: | Mouithys-Mickalad Ange Deby-Dupont Ginette Dogne Jean-Michel de Leval Xavier Kohnen Stephan Navet Rachel Sluse Francis Hoebeke Maryse Pirotte Bernard Lamy Maurice |
| |
Institution: | Centre for Oxygen, Research and Development (C.O.R.D.), Institut de Chimie, B6a, University of Liège, Sart Tilman, 4000 Liège, Belgium. amouithys@ulg.ac.be |
| |
Abstract: | Chronic inflammation through foam cells and macrophages is important in atherosclerosis development, and can be considered as therapeutic targets. Cyclooxygenase and NADPH-oxidase were expressed within atherosclerotic lesions. Reactive oxygen species produced by NADPH oxidase were found to trigger the cyclooxygenase-2 expression. The effects of preferential COX-2 inhibitors on ROS produced by Chlamydia-primed human monocytes (THP-1 cells) were evaluated by fluorescence, chemiluminescence, oxymetry, and EPR spin trapping. Fluorescence assays showed an increased production of ROS with Chlamydia versus cells primed by 10(-8)M PMA. COX-2 inhibitors inhibited in a dose-dependent manner the luminol-enhanced CL while ibuprofen and diclofenac increased the chemiluminescence response. By EPR spin trapping, COX-2 inhibitors, ibuprofen, and diclofenac, exhibited a dose-dependent inhibiting effect (10 and 100muM) on the EPR signal appearance. Our cell model combining EPR, chemiluminescence, and oxymetry appeared relevant to study the modulating effects of preferential COX-2 inhibitors on the cell oxidant activity and chronic inflammatory diseases. |
| |
Keywords: | Chlamydia pneumoniae Cyclooxygenase-2 enzyme NADPH-oxidase COX-2 inhibitors ROS Electron paramagnetic resonance Chemiluminescence Fluorescence Oxymetry |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|