Compositional Difference of the Exopolysaccharides Produced by the Virulent and Virulence-Deficient Strains of <Emphasis Type="Italic">Xanthomonas oryzae</Emphasis> pv. <Emphasis Type="Italic">oryzae</Emphasis> |
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Authors: | Ashok Kumar R Sunish Kumar N Sakthivel |
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Institution: | (1) Department of Biotechnology, Pondicherry University, Kalapet, Pondicherry 605014, India, IN |
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Abstract: | Xanthomonas oryzae pv. oryzae, the bacterial blight pathogen of rice, is known to produce phytotoxic polysaccharides. The extracellular polysaccharide (EPS)
was isolated from virulent (BXO1) and virulence-deficient gum G mutant (BXO1002) strains of X. oryzae pv. oryzae and characterized using fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR). Data from the FT-IR suggested
that the aldehyde (R-CHO) group and C=O of acid anhydride are present in BXO1 but absent in BXO1002. The 1H-NMR spectra showed the presence of an acetyl amine of hexose or pentose, free amines of glucose, an β-anomeric carbon of
hexose and pentose, hydrogen next to hydroxyl group, an acetyl amine of hexose and pentose in the polysaccharides of both
BXO1 and BXO1002, and the absence of α-anomeric carbon of hexose or pentose and the glucuronic acid in the polysaccharides
produced by BXO1002. The test for glucuronic acid also confirmed the absence of glucuronic acid in the polysaccharides of
BXO1002 and the presence glucuronic acid (32 μg/mg) in the polysaccharides produced by BXO1.
Received: 14 May 2002 / Accepted: 21 June 2002 |
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