Biosynthesis of triterpenoid sapogenols in soybean and alfalfa seedlings |
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Authors: | Irena Peri Uzi Mor Erich Heftmann Aron Bondi Yehuda Tencer |
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Affiliation: | 1. Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel;2. U.S. Department of Agriculture, Western Regional Research Center, Berkeley, California, U.S.A. |
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Abstract: | By incubation of germinating soybeans with mevalonate-[2-14C] (MVA), radioactivity was incorporated into four sapogenols which were identified by TLC. Unequivocal evidence for the identity of three of the four sapogenols was provided by co-crystallization to constant specific radioactivity. The partition of incorporated radioactivity into lipid- and water-soluble fractions and the pattern of radioactivity of individual sapogenols varied with the mode of administering labeled substrates to soybean seedlings, such as incubation of germinating soybeans with MVA-[2-14C], immersion of roots into MVA-[2-14C] or foliar application of squalene-[14C]. When alfalfa seedlings were incubated with MVA-[2-14C], about two-thirds of the radioactivity incorporated into the sapogenols was associated with medicagenic acid. |
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Keywords: | Leguminosae biosynthesis triterpenoid sapogenols. |
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