A review of the use of genetically engineered enzymes in electrochemical biosensors |
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| Authors: | Mònica Campàs Beatriz Prieto-Simón Jean-Louis Marty |
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| Institution: | 1. Department of Biochemistry, Faculty of Science, P. J. ?afárik University in Ko?ice, Moyzesova 11, 04154 Ko?ice, Slovakia;2. Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland;3. Department of Biophysics, P. J. ?afárik University in Ko?ice, Jesenná 5, 04154 Ko?ice, Slovakia;4. Biomedical Research Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 845 05 Bratislava, Slovakia;5. Institute of Microbiology of the CAS, v.v.i., Videnska 1083, 142 20 Prague 4, Czech Republic;6. Centre for Interdisciplinary Biosciences, P. J. ?afárik University in Ko?ice, Jesenná 5, 04154 Ko?ice, Slovakia |
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| Abstract: | This article gives an overview of the electrochemical biosensors that incorporate genetically modified enzymes. Firstly, the improvements on the sensitivity and selectivity of biosensors that integrate mutated enzymes are summarised. Next, new trends focused on the oriented immobilisation of mutated enzymes through specific functional groups located at their surface are reviewed. Finally, the effect of enzyme mutations on the electron transfer distance and kinetics of electrochemical biosensors is described. |
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