Antibody production,design and use for biosensor-based applications |
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| Authors: | Paul J Conroy Stephen Hearty Paul Leonard Richard J O’Kennedy |
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| Institution: | 1. Department of Electrical Engineering and Computer Science, the University of Tennessee, Knoxville, TN 37996, USA;2. Key Laboratory of Optoelectronic Technology and System of the Education Ministry of China, Chongqing University, Chongqing 400044, China;3. Department of Public Health, the University of Tennessee, Knoxville, TN 37996, USA;4. Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996, USA;1. Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Penang, Malaysia;2. OCRCC, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia;1. School of Biomedical Sciences, Huaqiao University, Fujian, 362000, China;2. The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Fujian, 362000, China;3. Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China |
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| Abstract: | Currently, the reliable detection and quantification of a multitude of different analytes is crucial in many applications and settings. Biosensors have revolutionised diagnostics for use in point-of-care testing (POC), the detection of food and environmental contaminants, biological warfare agents, illicit drugs and human/animal disease markers. Antibodies continue to play a pivotal role in many sensor devices due to their exquisite specificity for their cognate antigens. In this review current biosensor platforms employing antibodies for molecular recognition are briefly described. The use of molecular biological techniques for the generation and improvement of antibodies is critically examined. Such recombinant antibodies possess improved attributes for use in biosensor development in terms of design, stability, affinity and specificity. |
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