Fasudil Mesylate Protects PC12 Cells from Oxidative Stress Injury via the Bax-Mediated Pathway |
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Authors: | Qin?Li Dong?Liu Xianju?Huang Email author" target="_blank">Lianjun?GuoEmail author |
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Institution: | (1) Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;(2) People’s Hospital of Shanghai Jiao Tong University, Shanghai, China;(3) Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; |
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Abstract: | We previously reported that fasudil mesylate (FM) improves neurological deficit and neuronal damage in rats with ischemia
following middle cerebral artery occlusion and reperfusion in vivo. In this study, the properties of FM on hydrogen peroxide
(H2O2)-induced oxidative stress insult in cultured PC12 cells as well as the underlying mechanisms were investigated in vitro.
Pretreatment with FM (5, 10 μM) prior to H2O2 exposure significantly elevated cell viability, reduced cell apoptosis by MTT assay, LDH assay, Hoechst 33258 dye staining,
and FM also decreased the accumulation of reactive oxygen species (ROS) by DCFH-DA staining and NBT test. Furthermore, FM
also reversed the upregulation of Bax/Bcl-2 ratio, the downstream cascade following ROS. FM protected PC12 cells from oxidative
stress insult via down-regulating the Bax/Bcl-2 ratio. These findings indicate that a direct effect of fasudil mesylate on
PC12 cells may be partly responsible for its protective effect against oxidative stress injury. |
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