Substrate inhibition kinetics of <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> in fed-batch cultures operated at constant glucose and maltose concentration levels |
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Authors: | M Papagianni Y Boonpooh M Mattey B Kristiansen |
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Institution: | (1) Department of Hygiene and Technology of Food of Animal Origin, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, 54006, Greece;(2) Department of Bioscience, University of Strathclyde, Royal College Building, 204 George Street, Glasgow, G1 1XW, UK;(3) Present address: MediMush AS, MediMush Innovation Centre, 2970 Horsholm, Denmark |
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Abstract: | Fed-batch culture is the mode of operation of choice in industrial baker’s yeast fermentation. The particular mode of culture,
operated at stable glucose and maltose concentration levels, was employed in this work in order to estimate important kinetic
parameters in a process mostly described in the literature as batch or continuous culture. This way, the effects of a continuously
falling sugar level during a batch process were avoided and therefore the effects of various (stable) sugar levels on growth
kinetics were evaluated. Comparing the kinetics of growth and the inhibition by the substrate in cultures grown on glucose,
which is the preferential sugar source for Saccharomyces cerevisiae, and maltose, the most common sugar source in industrial media for baker’s yeast production, a milder inhibition effect by
the substrate in maltose-grown cells was observed, as well as a higher yield coefficient. The observed sugar inhibition effect
in glucostat cultures was taken into account in modeling substrate inhibition kinetics. The inhibition coefficient K
i increased with increasing sugar concentration levels, but it appeared to be unaffected by the type of substrate and almost
equal for both substrates at elevated concentration levels. |
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Keywords: | Saccharomyces cerevisiae Fed-batch culture Glucostat Substrate inhibition kinetics Glucose Maltose |
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