| 表达大肠杆菌LT-B/ST融合抗原的减毒鼠伤寒沙门氏菌株的构建 |
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| 引用本文： | 徐兵,舒东,张兆山,李淑琴,俞守义. 表达大肠杆菌LT-B/ST融合抗原的减毒鼠伤寒沙门氏菌株的构建[J]. 生物技术通讯, 1998, 0(4) |
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| 作者姓名： | 徐兵 舒东 张兆山 李淑琴 俞守义 |
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| 作者单位： | 第一军医大学，第一军医大学，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，第一军医大学 北京 100071，北京 100071 |
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| 基金项目： | 国家科技部863基金(课题号 863-102 07-03-05) |
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| 摘 要： | 编码LT-B/ST融合抗原的基因插入pYA248载体中,构建了重组质粒pXZL66。该重组质粒转入无毒鼠伤寒沙门氏菌SR-11,ΔCya,Δcrp,Δasd菌株X4072。此无抗药性的杂合菌株X4072(pXZL66)表达的LT-B/ST融合抗原具有LT和ST抗原性而没有生物毒性,可望成为预防ETEC腹泻和相应的沙门氏菌病双价口服活疫苗候选株。
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| 关 键 词： | 产肠毒素大肠杆菌 热敏肠毒素B亚基 耐热肠毒素 鼠伤寒沙门氏菌 |
Construction of the attenuated Salmonella typhimurium strain expressing Escherichia coli LT-B/ST fusion antigens |
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| Xu Bing,Shu Dong,Zhang zhaoshan,Li Shuqin,Yu Shouyi. Construction of the attenuated Salmonella typhimurium strain expressing Escherichia coli LT-B/ST fusion antigens[J]. Letters in Biotechnology, 1998, 0(4) |
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| Authors: | Xu Bing Shu Dong Zhang zhaoshan Li Shuqin Yu Shouyi |
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| Abstract: | The fusion gene encoding LT-B and ST antigens was inserted into the vector plasmid pYA248. The resulting recombinant plasmid pXZL66 was transformed into the delta cya delta crp delta asd attenuated Salmonella ty-phimurium vaccine strain X4072, and the live vaccine strain X4072(pXZL66) was constructed. Meanwhile, LT B/ST fusion antigen expressed by X4072 (pXZL66) was no biological toxicity. This live vaccine strain X4072(pXZL66) is worth considering as a new bivalent live oral vaccine candidate against ETEC diarrhea and relative Salmonella disease. |
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| Keywords: | ETEC heat-labile enterotoxin B subunit heat-stable enterotoxin S. typhimurium |
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